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KMID : 1134820160450081099
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 8 p.1099 ~ p.1106
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Immunomodulatory Activity of Crude Polysaccharide Separated from Cudrania tricuspidata Leaf
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Byun Eui-Baek
Jang Beom-Su
Sung Nak-Yun
Byun Eui-Hong
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Abstract
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The objective of this study was to evaluate the immunomodulatory activity of crude polysaccharide separated from Cudrania tricuspidata leaf. C. tricuspidata polysaccharide (CTP) was extracted by ethanol precipitation. Immunomodulation activity was tested in macrophage cells (RAW 264.7 and bone-marrow derived macrophage) and splenocytes. CTP treatment significantly increased cell proliferation up to 250 μg/mL in both RAW 264.7 and bone-marrow derived macrophages. In this concentration range (below 250 μg/mL), nitric oxide and cytokine [tumor necrosis factor (TNF)-α and interleukin (IL)-6] production also significantly increased. Similarly, splenocyte proliferation dose- dependently increased except for the 1,000 μg/mL treated group. Regarding cytokine production activity in splenocytes, CTP treatment significantly increased production of Th 1 type cytokines [interferon (IFN)-γ] production but not Th 2 type cytokines (IL-4). Therefore, the results indicate that CTP may have a potential effect on immunomodulatory activity in various immune cells, and this is useful for development of immune enhancing adjuvant materials as a natural ingredient.
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KEYWORD
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Cudrania tricuspidata, polysaccharide, immunomodulatory activity, macrophage cells, splenocyte
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